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C-Peptide, EIA ,Kit, for serum, plasma, culture supernatant, and cell lysates Catalog Number RAB0326 Storage Temperature –20 C TECHNICAL BULLETIN Product Description 8. The ,C-Peptide, Enzyme Immunoassay (EIA) ,Kit, is an in vitro quantitative assay for detecting ,C -Peptide, based on the principle of competitive enzyme immunoassay.
A standard curve of known concentration ,of C-Peptide, peptide can be established and the concentration ,of C-Peptide, peptide in the samples can be calculated accordingly. EIA-CPE-1 only detects ,C-peptide,, not Insulin A or B chain. The minimum detectable concentration ,of C-Peptide, is 772 pg/ml. The ,detection, range for the ,kit, is 0.1-1,000 ng/ml.
methods. Here, a commercially available sandwich ELISA ,kit, with modified components was used to quantify ,C-peptide, levels in human serum samples. Overview Of Method ,Development, 50 µL of sample, together with Biotin ... ,Development, of a Highly Sensitive Assay for the ,Detection, of the Biomarker ,C-Peptide, in Human Serum M. Hyvönen, M ...
Human ,C-Peptide, has a molecular mass of approximately 3000 daltons. ,C-Peptide, has no metabolic function. However, since ,C-Peptide, and insulin are secreted in equimolar amounts, the immunoassay ,of C-Peptide, permits the quantitation of insulin secretion. This is the reason for the clinical interest of serum or plasma determinations ,of C- Peptide,.
ELISA ,kits, for PIP ,detection, The Procollagen Type I ,C-Peptide, (PIP) EIA ,Kit, (Precoated) is a 96-well format in vitro enzyme immunoassay intended for the quantification of human, bovine, canine, and horse PIP in plasma, serum, cell culture extract, cell culture supernatant, and other biological fluids.
C-peptide, bound in the initial step. The color ,development, is stopped and the intensity of the color is measured. ,DETECTION, RANGE 1.25 ng/ml-80 ng/ml. SENSITIVITY The minimum detectable dose of pig ,C-peptide, is typically less than 0.312 ng/ml. The sensitivity of this assay, or Lower Limit of ,Detection, (LLD) was defined as
The peptide sequence ERMRPRKRQGSVRRRV corresponds to the pseudosubstrate region of the ε-isotype of protein kinase C (PKCε) with an alanine to serine substitution. PKCε exhibits an apparent specificity for the native peptide (Km=68 µM).
Peroxidase (HRP) conjugated antibody specific for ,C-Peptide,. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount ,of C-Peptide, bound in the initial step. The color ,development, is stopped and the intensity of the color is measured. ,DETECTION, RANGE 0.7 ng/ml-10 ...
C-Peptide, levels are in many ways a better measurement of endogenous insulin secretion than peripheral insulin levels. ,C-Peptide, may be measured in either blood or urine. With improved sensitive ,C-Peptide, immunoassays, it is now possible to measure ,C-Peptide, values at extremely low levels.